Biosynthesis, Purification and Characterization of Cadmium Sulfide Nanoparticles Using Enterobacteriaceae and their Application

The area of nanotechnology encompasses the synthesis of nanoscale materials, the understanding and the utilization of their physicochemical and optoelectronic properties, and the organization of nanoscale structures into predefined superstructures. The development of biologically inspired experimental processes for the synthesis of nanoparticles is evolving into an important branch of nanotechnology. Nanotechnology has recently emerged as an elementary division of science and technology that investigates and regulates the interaction at cell level between synthetic and biological materials with the help of nanoparticles. A wide range clean, nontoxic and eco-friendly synthesis of nanoparticles is an important aspect of current nanotechnology. Microbial synthesis of nanoparticles is a Green chemistry approach that interconnects nanotechnology and microbial biotechnology. Microorganisms play an important role in the eco-friendly synthesis of metal nanoparticles. This study illustrates the synthesis of CdS nanoparticles using the bacteria of Enterobacteriaceae ( Escherichia coli PTCC 1533 and Klebsiella pneumonia PTCC 1053) after 96 h of incubation at room temperature (30oc) and pH 9. The morphology of the samples was analyzed using Scanning electron microscopy(SEM). The size of CdS nanoparticles in aqueous solution has been calculated using UV–Vis spectroscopy, XRD, FTIR, EDS and SEM measurements. The nanoparticles are found to be polydisperse in the size range 5–200 nm.